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目的对14株上海市崇明地区2005年O139群霍乱弧菌菌株进行脉冲场凝胶电泳(PFGE)及核糖体分型分析。方法用PFGE、核糖体分型对霍乱弧菌进行分析。结果 14株O139群霍乱弧菌产毒株经核糖体分型分析为同一型;而PFGE双酶切分型结果为其中10株为同一型,其余4株为高度同源性。结论上海市崇明地区2005年流行的O139群霍乱弧菌核酸指纹分型基本一致。
Objective To analyze the genotypes of 14 isolates of Vibrio cholerae O139 from Chongming district of Shanghai in 2005 by pulsed-field gel electrophoresis (PFGE) and ribosome typing. Methods Vibrio cholerae was analyzed by PFGE and ribosome typing. Results The 14 isolates of Vibrio cholerae O139 were identified as the same type by ribosome typing. The results of PFGE digestion showed that 10 isolates belonged to the same type and the remaining 4 strains were highly homologous. Conclusion The fingerprinting of Vibrio cholerae O139 strains in Chongming area in 2005 was basically the same.