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荞麦基因资源相对匮乏。以甜荞根和金荞根为材料,利用Illumina Hi SeqTM2000对其进行转录组测序,经过de novo从头组装得到64 618条Unigene,其中37 546条基因得到了有效注释。对甜荞根和金荞根中的差异基因进行筛选,共获得了4 709个差异基因,其中2 460个上调表达,2 249个下调表达。这些差异基因的GO注释集中在10个细胞组分(cellular component)、12个分子功能(molecular function)和22个生物学过程(biological process)中。对差异基因参与的代谢途径进行富集,上调基因中共有40个代谢途径显著富集,下调基因中共有18个代谢途径显著富集,这些显著富集的代谢途径参与甜荞根和金荞根的生物学调控。最后对转录组中注释到黄酮合成途径中的关键基因进行了分析,共有21个基因注释到黄酮合成途径中的关键酶。不仅丰富了荞麦的基因资源,为荞麦分子生物学研究提供数据基础,也为筛选甜荞根和金荞根中相关基因的表达趋势提供参考依据。
Buckwheat gene resources are relatively scarce. A total of 64 618 Unigene were obtained by de novo assembly, of which 37 546 genes were effectively annotated by transcriptome sequencing using Illumina Hi SeqTM2000. A total of 4 709 differentially expressed genes were screened for the differential genes in sweet and blue buckthorn roots, of which 2 460 were up-regulated and 2 249 down-regulated. The GO annotations for these differential genes are focused on 10 cellular components, 12 molecular functions and 22 biological processes. A total of 40 metabolic pathways were significantly enriched in the up-regulated genes. A total of 18 metabolic pathways were significantly enriched in the down-regulated genes. These significantly enriched metabolic pathways involved in the biogenesis of the sweet and blue buckthorn roots Learning regulation. At last, we analyzed the key genes annotated to the flavonoid synthesis pathway in the transcriptome, and a total of 21 genes were annotated the key enzymes in flavonoid biosynthesis pathway. It not only enriches the gene resources of buckwheat, provides the data basis for the molecular biology research of buckwheat, but also provides a reference for screening the expression trends of the related genes in the roots of S. tibetanum and S. japonicum.