论文部分内容阅读
探讨头部重点低温（ＳＨＣ）能否改善受损兔脑神经元的转归，以及相应的生化机制。方法：采用“四血管”模式造成脑缺血３０ｍｉｎ，然后将１６８只新西兰兔随机分为三组，Ⅰ组为未缺血对照组；其余两组动物的３个亚组均在缺血３０ｍｉｎ后分别再灌注３０、１８０和３６０ｍｉｎ；Ⅱ组为常温再灌注组；Ⅲ组为ＳＨＣ治疗组，开始再灌注时实施ＳＨＣ（２８℃）。检测了脑组织中２１项生化指标的变化和采用计算机图像分析技术，将１２个脑区的神经元分为Ａ（正常）、Ｂ（轻伤）、Ｃ（重伤）和Ｄ（坏死）四类。采用ＳＰＳＳ作相关分析和ｔ检验。结果：与Ⅰ组相比，Ⅱ组Ａ类神经元所占百分比在再灌注时进行性下降，Ｂ、Ｃ类明显增多，Ｄ类则进行性增多（均Ｐ＜０．０１）；与Ⅱ组相比，Ⅲ组Ａ类进行性增多，Ｂ类也增多；Ｃ、Ｄ类减少（均Ｐ＜０．０１）；Ⅱ组与Ａ类改变相关的生化指标是ＶＩＰ、β－ＥＰ、ＰＧＩ２、Ｔ３、Ｔ４和Ｎａ＋－Ｋ＋ＡＴＰ酶；与Ｂ类是β－ＥＰ和ＴＸＢ２；与Ｃ类是Ｇｌｎ、ＴＸＢ２／ＰＧＩ２；与Ｄ类是Ｔ４、Ｎａ＋－Ｋ＋ＡＴＰ酶、Ｇｌｎ、Ｔ３和ＶＩＰ（Ｐ＜０．０５）。Ⅲ组Ｃａ２＋－Ｍｇ２＋ＡＴＰ酶与Ａ、Ｃ和Ｄ类变化相关（Ｐ＜０．０５）。结论：ＳＨＣ确能改善受损神经?
To investigate whether head-key hypothermia (SHC) can improve the neuronal outcome of injured rabbit brain and its corresponding biochemical mechanism. Methods: The model of “four vessels” was used to induce cerebral ischemia for 30 minutes. 168 New Zealand rabbits were randomly divided into three groups. Group Ⅰ was not ischemic control group. The other three subgroups of the two groups were given 30 min after ischemia Then reperfused for 30, 180 and 360 minutes respectively; group Ⅱ was normal temperature reperfusion group; group Ⅲ was SHC treatment group, and SHC (28 ℃) was started when reperfusion started. The changes of 21 biochemical indexes in brain tissue were detected and the neurons in 12 brain regions were divided into A (normal), B (minor), C (severe) and D (necrosis) . SPSS was used for correlation analysis and t test. Results: Compared with group Ⅰ, the percentage of type A neurons in group Ⅱ decreased progressively at reperfusion, while group B and C increased significantly, while group D increased progressively (all P <0.01) Compared with group A, the number of group A increased progressively, the number of group B increased, while the number of class C and D decreased (all P <0.01). The biochemical indexes of group Ⅱ related to type A change were VIP, β-EP, PGI2, T3 , T4 and Na + -K + ATPase; and class B was β-EP and TXB2; group C was Gln and TXB2 / PGI2; and group D was T4, Na + -K + ATPase, Gln, T3 and VIP ). The level of Ca2 + -Mg2 + ATPase in group Ⅲ was correlated with changes of A, C and D (P <0.05). Conclusion: SHC can really improve the damaged nerve?