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[目的]检测腹泻型肠易激综合征(IBS-D)患者结肠组织5羟色胺3受体mRNA(5-HT3R mRNA)的表达水平,探讨中药人参调脾散治疗IBS-D的作用机制。[方法]采用逆转录-聚合酶链式反应(RT-PCR)方法分析IBS-D患者治疗前后及正常人(对照组)乙状结肠黏膜5-HT3R mRNA表达水平的变化。[结果]IBS-D患者治疗前5-HT3R mRNA表达水平较对照组显著增高,2组比较差异有统计学意义(P<0.05);经人参调脾散治疗后5-HT3R mRNA表达水平减低,与对照组比较差异无统计学意义(P>0.05);IBS-D患者治疗前后5-HT3R mRNA表达水平自身比较差异有统计学意义(P<0.05)。[结论]人参调脾散能通过下调IBS-D患者结肠黏膜5-HT3RmRNA表达,降低5-HT3R活性,达到治疗IBS-D的作用。
[Objective] To investigate the expression of 5-HT3R mRNA in colon tissue of patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and to explore the mechanism of action of Ginseng Tiao-Pi Tiao for the treatment of IBS-D. [Methods] The changes of 5-HT3R mRNA expression in sigmoid colon mucosa in IBS-D patients before and after treatment and in normal people (control group) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) [Results] The expression of 5-HT3R mRNA in IBS-D patients before treatment was significantly higher than that in the control group (P <0.05). The expression of 5-HT3R mRNA in IBS- Compared with the control group, there was no significant difference (P> 0.05). The expression level of 5-HT3R mRNA in IBS-D patients before and after treatment had statistically significant difference (P <0.05). [Conclusion] Ginseng Tiao-sixian can reduce the 5-HT3R mRNA expression in colonic mucosa of IBS-D patients and reduce the activity of 5-HT3R to treat IBS-D.