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【目的】大豆疫霉菌指纹分析的建立和黑龙江与新疆大豆疫霉菌群体的群体遗传分析。【方法】利用生物信息学方法寻找大豆疫霉菌(Phytophthora sojae)的中度重复序列,并对黑龙江和新疆大豆疫霉菌进行DNA指纹分析。【结果】分析得到一个中度重复序列,定名为PS1227。Southern blot分析表明PS1227在大豆疫霉菌基因组中约有34条可辨的介于1.5-23kb之间的杂交条带,其中21个杂交条带在49个供试菌系中表现多态性。单游动孢子分析表明PS1227指纹特征在病菌无性生殖阶段表现稳定。利用PS1227标记,本实验发现采自黑龙江HP4002、SY6和GJ0105菌系分别与新疆的DW303、71228和71222菌系具有完全相同的指纹特征。【结论】获得一个可用于大豆疫霉菌流行学和群体生物学研究的指纹分析序列PS1227,在分子水平证实了新疆大豆疫霉菌可能由黑龙江传入。
【Objective】 Fingerprinting of Phytophthora sojae and population genetic analysis of Phytophthora sojae in Heilongjiang and Xinjiang were carried out. 【Method】 The bioinformatics method was used to search for moderate repeats of Phytophthora sojae. DNA fingerprinting of Phytophthora sojae in Heilongjiang and Xinjiang was carried out. 【Result】 A moderate repeat was obtained and named as PS1227. Southern blot analysis showed that there are about 34 discernible bands of 1.5-23 kb in PS1227 genome of Phytophthora sojae, of which 21 hybrids showed polymorphism in 49 tested strains. Monotumatic zoospore analysis showed that PS1227 fingerprinting was stable during the vegetative stage of the germ. Using PS1227 marker, we found that the strains of HP4002, SY6 and GJ0105 collected from Heilongjiang Province had the same fingerprints as DW303, 71228 and 71222 strains of Xinjiang respectively. 【Conclusion】 A fingerprinting sequence PS1227, which can be used for the epidemiology and population biology of Phytophthora sojae, was obtained at the molecular level and confirmed that Phytophthora sojae from Xinjiang may be introduced from Heilongjiang.