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利用来自假单胞菌的ＧＬ－７－ＡＣＡ酰化酶的信号肽和表达元件基因片段构建了ＧＬ－７－ＡＣＡ酰化酶的分泌型高表达质粒ｐＴｒｃＣＡ１Ｓ和ｐＫＫＣＡ１Ｓ，其中ｐＴｒｃＣＡ１Ｓ为ＩＰＴＧ诱导型质粒，ｐＫＫＣＡ１Ｓ为组成型质粒。ｐＴｒｃ－ＣＡ１Ｓ和ｐＫＫＣＡ１Ｓ转入受体菌ＴＧ１中都可高表达ＧＬ－７－ＡＣＡ酰化酶基因并将表达产物转运到周质空间，完整细胞酰化酶比活力分别为２３．９单位每克菌体和１８．３单位每克菌体。分别为原分泌型表达质粒ｐＭＲ２４的ＴＧ１转化子完整细胞酰化酶比活力的８倍和６倍左右
The secreted high expression plasmids pTrcCA1S and pKKCA1S of GL-7-ACA acylase were constructed using the signal peptide and expression element gene fragments of GL-7-ACA acylase from Pseudomonas, in which pTrcCA1S is an IPTG inducible plasmid , PKKCA1S is a constitutive plasmid. The transfection of pTrc-CA1S and pKKCA1S into the recipient strain TG1 overexpressed the GL-7-ACA acylase gene and transported the expressed product to the periplasmic space. The specific activity of intact cell acylase was 23.9 units per gram Cells and 18.3 units per gram of bacteria. Respectively, the original secreting plasmid pMR24 TG1 transformant intact cell acylase specific activity of 8 times and 6 times