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采用受体放射配体结合技术测定了 5 0例正常健康成人、18例健康儿童、5 0例急性淋巴细胞白血病 (ALL )患者和 33例肾病综合症 (NS)患者外周血淋巴细胞糖皮质激素高亲和力受体 GCRH 和低亲和力受体 GCRL 位点数 ,分析 GCR与疗效的关系 ;对 NS患者还检测了血浆 ACTH、皮质醇水平 ,探讨了 NS发病及疗效机制 ;同时还通过动物实验观察了中药名方小柴胡汤对应用 GC所产生的 GCR“降调节”效应的影响。结果表明 :AL L患者治疗前 GCRH>4 0 0 0位点 /细胞的患者对激素联合化疗疗效好 ,AL L患者在激素联合化疗后 GCRH 明显降调 ,但 GCRL 治疗前后无明显变化 ;NS患者治疗前 GCRH>6 0 0 0位点 /细胞的患者对 GC疗效好 ,GCRH<30 0 0位点 /细胞的患者对 GC治疗无效 ;所以对 AL L、NS患者疗前了解 GCR水平 ,有助于制定个体化的治疗措施。 SD大白鼠实验研究表明 ,小柴胡汤具有调控 GCR的作用
The receptor radioligand binding technique was used to detect the levels of peripheral blood lymphocyte glucocorticoids in 50 normal healthy adults, 18 healthy children, 50 acute lymphoblastic leukemia (ALL) patients and 33 nephrotic syndrome (NS) High affinity receptor GCRH and low affinity receptor GCRL sites, analysis of the relationship between GCR and efficacy; NS patients were also tested for plasma ACTH, cortisol levels, explore the pathogenesis and efficacy of NS mechanism; also observed by animal experiments of traditional Chinese medicine Effect of Fang Xiaochaihu Decoction on “Down-regulation” Effect of GCR Produced by GC. The results showed that: AL L patients before treatment GCRH> 400 sites / cells in patients with hormone combination chemotherapy, AL L patients after hormone combination chemotherapy GCRH significantly decreased, but no significant changes before and after GCRL treatment; NS patients Patients with pre-treatment GCRH> 600 sites / cells had good GC responses and patients with GCRH <30 000 sites / cells had no effect on GC treatment; therefore, understanding GCR levels prior to treatment in patients with AL and NS may be helpful In the development of individualized treatment. SD rat experimental study shows that Xiao Chai Hu Tang has the role of regulation of GCR