确定美伐他汀产生菌橘青霉(Penicillium citrinum)的原生质体的制备及再生的最佳条件.通过正交实验设计的方法,确定了接种量约108个孢子时,于85 mL YPL-20液体培养基中25℃,210 rpm,培养60 h,0.5%纤维素酶和蜗牛酶等比例混合酶液,在30℃酶解2 h,以蔗糖为高渗稳定剂的PGA培养基,原生质体再生形成率可达42.45%.本结果为深入研究橘青霉菌株的原生质体诱变、构建基因工程菌和提高美伐他汀产量奠定基础. The optimum conditions for the preparation and regeneration of protoplasts of meiguasan-producing Penicillium citrinum were determined.According to the method of orthogonal experimental design, when the amount of inoculum was about 108 spores, it was determined that 85 mL of YPL-20 liquid The medium was incubated at 25 ℃, 210 rpm for 60 h, mixed with 0.5% cellulase and snail enzyme, enzymatically hydrolyzed at 30 ℃ for 2 h, and sucrose as a hypertonic stabilizer in PGA medium. Protoplast regeneration The formation rate of up to 42.45% .This result for in-depth study of protoplast mutagenesis Penicillium strains to build genetic engineering bacteria and improve the production of mevastatin basis.