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目的通过分析SPF级雄性自发性高血压(SH)大鼠和WKY大鼠的肺泡灌洗液成分、观察动物的一般指标,肺组织的病理学特征,研究正常血压对照组大鼠和自发性高血压模型组大鼠的肺部血管损伤及肺部实质细胞损伤的差异。方法将48只SPF级雄性SH大鼠和WKY大鼠随机分为4组,每组6只,分别为生理盐水对照组及低、中和高剂量组,采用气管灌注的方法进行染毒,收集肺灌洗液,分析肺泡灌洗液成分并观察肺组织的病理切片。结果染毒组动物的体质量与染毒前比较差异均有统计学意义(P<0.05),并且中、高剂量染毒组动物与染毒前比较均降低,差异有统计学意义(P<0.05);染毒组动物的呼吸与染毒前比较均增快,差异具有统计学意义(P<0.05);染毒组肺泡灌洗液白蛋白(ALB)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)及乳酸脱氢酶(LDH)的水平与对照组比较差异均有统计学意义(P<0.05),SHR高剂量组动物肺泡灌洗液中上述指标的水平高于相同剂量下的WKY,差异具有统计学意义(P<0.05)。通过病理学观察发现染毒后动物肺部组织有明显病理性改变。结论细颗粒物对大鼠肺部有损伤作用,而SHR比WKY对大气细颗粒物致肺损伤的作用更加易感。因此、高血压疾病可能是大气细颗粒物引起肺部损伤的危险因素之一。
OBJECTIVE: To analyze the composition of bronchoalveolar lavage fluid (BALF) in male spontaneously hypertensive rats (SH) and WKY rats, and to observe the general characteristics of the animals and the pathological features of lung tissue in normal rats. Differences of pulmonary vascular injury and pulmonary parenchymal injury in blood pressure model rats. Methods Forty-eight SPF male SH rats and WKY rats were randomly divided into 4 groups, 6 rats in each group, which were saline control group and low, medium and high dose groups, respectively. Tracheal perfusion was performed to collect Lung lavage fluid, analysis of alveolar lavage fluid composition and observation of lung tissue biopsy. Results The body weight of the exposed animals was significantly lower than that before exposure (P <0.05), and the animals in the medium and high dose groups were significantly lower than those before exposure (P < 0.05). The respiration of the exposed animals was faster than that before exposure, and the difference was statistically significant (P <0.05). The levels of albuminuria (ALB), alkaline phosphatase (AKP) The levels of acid phosphatase (ACP) and lactate dehydrogenase (LDH) were significantly different from those of the control group (P <0.05). The levels of above indicators in the high-dose SHR group were higher than those of the same dose Under WKY, the difference was statistically significant (P <0.05). Pathological observation found that the pathological changes of lung tissue after exposure to animals. CONCLUSIONS: Fine particles have a damaging effect on the lungs of rats, whereas SHR is more susceptible to the effects of WKY on lung injury induced by fine particles of the atmosphere. Therefore, hypertensive diseases may be one of the risk factors of lung damage caused by fine particles of the atmosphere.