论文部分内容阅读
作为重要的天然药物产物,姜黄素(Curcumin)分离自Curcuma longa L.,具有多个方面的活性,包括抗氧化、抗感染和抗炎作用。最近的研究提示姜黄素可能具有一定的抗肿瘤活性,但其活性和作用机制有待深入研究。为考察姜黄素对抗肿瘤药物杀伤肺癌细胞的影响,用肺癌细胞系A549、H460、H1299以及H358使用姜黄素预处理后,分别使用抗肿瘤药物吉非替尼(Gefitinib)、舒尼替尼(Sunitinib)、吉西他滨(Gemcitabine)和紫杉醇(Paclitaxel)处理上述肺癌细胞,检测其抑制率;并计算IC50值。CCK-8实验结果显示,姜黄素能够上调抗肿瘤药物对肺癌细胞的杀伤作用,显著下调其IC50值。说明姜黄素具有增加肿瘤细胞对化疗药物敏感性的作用,具有潜在逆转化疗耐药的价值。
As an important natural drug product, Curcumin is isolated from Curcuma longa L. and has various activities including antioxidant, anti-infective and anti-inflammatory effects. Recent studies suggest that curcumin may have some anti-tumor activity, but its activity and mechanism need further study. In order to investigate the effect of curcumin on anti-tumor drug-killing of lung cancer cells, antitumor drugs Gefitinib, Sunitinib (Sunitinib) were respectively used to pretreat lung cancer cell lines A549, H460, H1299 and H358 with curcumin ), Gemcitabine and Paclitaxel, and the inhibition rate was measured. IC50 values were calculated. CCK-8 experimental results show that curcumin can up-regulate anti-tumor drugs on lung cancer cell killing effect, significantly down its IC50 value. Curcumin has the effect of increasing the sensitivity of tumor cells to chemotherapeutic drugs and has the potential to reverse the resistance of chemotherapy.