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目的对SLE患者外周血中单核细胞诱导,培养树突状细胞,并鉴定表面标志,分析DC表面标志与疾病活动指数之间的的相关性。方法采用密度梯度离心的方法分离外周血单核细胞,在培养瓶中贴璧3h,然后吸去悬浮细胞,联合应用GM-CSF,IL-4和TNF-α诱导分化,刺激正常人及SLE患者外周血DC增殖、分化、成熟。用免疫荧光标记培养的细胞,流式细胞仪分析DC各亚型,分析患者SLEDAI与DC各表型的相关性。结果SLE患者DC表达的CD1a,CD11c+,CD40和CD123百分率(58.88±7.64、54.4±10.88、37.29±8.08、13.14±4.44)较对照组(47.71±4.01、43.12±8.82、28.59±7.07、9.85±3.97)明显增加(P<0.05),SLE患者DC表达的CD80和CD83(55.16±10.12、57.76±11.54)较对照组(47.95±12.21、48.31±8.79)升高不明显(P>0.05)。SLEDAI与CD1a,CD11c+,CD40和CD123+呈明显相关性(P<0.05),与CD80和CD83相关性不明显。结论SLE患者DC表型表达增加,与疾病活动程度有密切关系。
OBJECTIVE: To induce and culture dendritic cells in mononuclear cells of peripheral blood of patients with SLE and to identify the surface markers. The correlation between DC surface markers and disease activity index was analyzed. Methods Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation. The cells were resuspended in culture flasks for 3 hours and then suspended in suspension cells. GM-CSF, IL-4 and TNF-α were used to induce differentiation and stimulate normal and SLE patients Proliferation, differentiation and maturation of peripheral blood DC. Cultured cells were labeled by immunofluorescence, DC subtypes were analyzed by flow cytometry, and the correlation between SLEDAI and each phenotype of DC was analyzed. Results The percentage of CD1a, CD11c +, CD40 and CD123 expression in SLE patients was significantly higher than that in control group (47.71 ± 4.01,43.12 ± 8.82,28.59 ± 7.07,9.85 ± 3.97 ) (P <0.05). The expression of CD80 and CD83 in SLE patients was significantly lower than that in the control group (47.95 ± 12.21, 48.31 ± 8.79) (P <0.05). SLEDAI was significantly associated with CD1a, CD11c +, CD40 and CD123 + (P <0.05), but not with CD80 and CD83. Conclusion SLE patients with DC phenotype expression increased, and the degree of disease activity are closely related.