论文部分内容阅读
目的观察经冠脉联合导入骨髓间充质干细胞(MSCs)及碱性成纤维细胞生长因子(bFGF)基因对急性心梗心功能的影响。方法开胸结扎法制作小猪急性心肌梗塞模型(11只),1周后行冠状动脉造影,随机分为对照组和实验组,分别经冠脉导入生理盐水10ml,含1×107MSCs的生理盐水10ml和pcDNA3-bFGF2000ug。于心梗前、心梗后1周以及心梗后5周行心脏超声检查,心梗后5周再次测定左室舒张末压。结果所有动物均成功建立急性心肌梗死模型,心梗后1周左室射血分数明显下降,5周后实验组心功能较对照组改善。结论联合导入骨髓间充质干细胞及碱性成纤维细胞生长因子基因可改善急性心肌梗死的心功能。
Objective To observe the effect of transfection of bone marrow mesenchymal stem cells (MSCs) and basic fibroblast growth factor (bFGF) gene on the cardiac function in patients with acute myocardial infarction (AMI). Methods Thirteen piglets with acute myocardial infarction were established by thoracotomy. One week later, coronary angiography was performed. The rats were randomly divided into control group and experimental group. The rats were infused with 10 ml normal saline, 10 ml normal saline containing 1 × 107 MSCs, pcDNA3-bFGF2000ug. Cardiac echocardiography was performed before myocardial infarction, 1 week after myocardial infarction, and 5 weeks after myocardial infarction. Left ventricular end-diastolic pressure was measured again 5 weeks after myocardial infarction. Results All the animals were successfully established acute myocardial infarction model, 1 week after myocardial infarction left ventricular ejection fraction decreased significantly after 5 weeks in the experimental group than in the control group improved cardiac function. Conclusion Combined introduction of BMSCs and basic fibroblast growth factor gene can improve cardiac function in patients with acute myocardial infarction.