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我院于1991年收治1例血管活性肠肽瘤(VIP瘤)患者,对其手术切除后病理标本应用免疫组化方法进行研究,并结合国内外VIP瘤的研究结果,以探讨其内分泌功能。 材料与方法 1.临床和病理材料 患者,女,43岁,手术及病理证实为胰头部良性VIP瘤,行胰头十二指肠切除术后痊愈出院。其手术切除标本经10％福尔马林固定,石蜡包埋。 2.试剂及其稀释度 9种第一抗体分别抗胰岛素(Ins,1:50)、胰高糖素(Glu,1:50)、胃泌素(Gas,1:50)、生长抑素(SS,1:50)、血管活性肠
In our hospital, in 1991, one patient with vasoactive intestinal peptide tumor (VIP tumor) was treated. Histopathological specimens after surgical resection were studied by immunohistochemical methods, and combined with the results of domestic and foreign VIP tumors to explore the endocrine function. Materials and Methods 1. Clinical and pathological materials Patients, females, 43 years old, surgically and pathologically confirmed as pancreatic head benign VIP tumors, pancreaticoduodenectomy was cured and discharged. The surgical specimens were fixed in 10% formalin and embedded in paraffin. 2. Reagents and their dilutions The 9 primary antibodies were insulin resistant (Ins, 1:50), glucagon (Glu, 1:50), gastrin (Gas, 1:50), and somatostatin ( SS, 1:50), vasoactive intestinal