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一、噬菌体的繁殖我們試驗室所使用的噬菌体为4832福氏及714宋内氏噬菌体。取500毫升完全澄清的肉膏湯或鲜肉湯加入噬菌体母液0.2毫升,再加入經过鑑定的与噬菌体相对应的光滑型菌株4—6小时培养菌液2毫升,置于37℃溫箱培养4—6小时,取出置于56℃—58℃水箱30分钟灭活,测定效价后,保存于冰箱内备用。二、指示噬菌体的效价测定 1.試管稀释法: (一) 取11支4.5毫升肉膏湯(或肉湯)管,排在試管架上,按順序写上10~(-1),……10~(-10),第11管作为对照。
First, the phage breeding Our laboratory phage used for 4832 Freesia and 714 Song Neike’s phage. Take 500 ml of completely clarified meat extract soup or fresh broth added to 0.2 ml phage stock solution, and then added to the identified phage smooth strains corresponding to 4-6 hours culture solution 2 ml, placed in 37 ° C incubator 4-6 hours, remove the water tank at 56 ℃ -58 ℃ inactivated 30 minutes, measured titer, stored in the refrigerator spare. Second, indicating the phage titer 1. Tube dilution method: (A) Take 4.5 ml of 4.5 ml meat soup (or broth) tube, arranged in a test tube rack, write in order 10 ~ (-1), ... ... 10 ~ (-10), the first 11 tubes as a control.