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目的探讨水杨酸钠对胰岛素抵抗大鼠胰岛素敏感性的影响及作用机制。方法分别给大鼠静脉输注脂肪乳+肝素,脂肪乳+肝素+水杨酸钠和生理盐水7 h,部分大鼠在输液的最后2 h同时行清醒状态高胰岛素-正血糖钳夹试验,测定血浆葡萄糖、游离脂肪酸(FFA)、胰岛素、C肽、丙二醛(MDA)水平,检测肝脏、肌肉中MDA含量及谷胱甘肽过氧化物酶(GSH-PX)活性。结果脂肪乳输注组葡萄糖输注率(GIR)是生理盐水输注组的45%,输注水杨酸钠可使GIR提高1.3倍(P<0.01)。与生理盐水输注组比较,脂肪乳输注组血浆、肝脏、肌肉中MDA水平增加了2~4倍(P<0.01);GSH-PX活性降低45%~50%(P<0.01)。水杨酸钠输注使MDA水平较脂肪乳输注组降低了62%~66%(P<0.01),GSH-PX活性升高35%~38%(P<0.05)。结论FFA增高引起机体氧化应激增强,可能是导致胰岛素抵抗发生的机制之一。应用水杨酸钠后大鼠氧化应激减弱,胰岛素抵抗改善,故水杨酸钠可能通过降低氧化应激途径而发挥改善胰岛素抵抗的作用。
Objective To investigate the effect of sodium salicylate on insulin sensitivity in insulin resistance rats and its mechanism. Methods Rats were given intravenous infusion of fat emulsion, heparin, fat emulsion, heparin, sodium salicylate and normal saline for 7 hours respectively. Some rats in the last 2 hours of transfusion simultaneously underwent awake high insulin - Plasma glucose, free fatty acid (FFA), insulin, C-peptide, and malondialdehyde (MDA) levels were measured and MDA content and glutathione peroxidase (GSH-PX) activity in liver and muscle were measured. Results Glucose infusion rate (GIR) was 45% in saline infusion group, and GIR increased 1.3 times (P <0.01) by infusion of sodium salicylate. Compared with saline infusion group, MDA level in plasma, liver and muscle increased 2 to 4 times (P <0.01) and GSH-PX activity decreased 45% to 50% (P <0.01). Sodium salicylate infusion decreased MDA levels by 62% -66% (P <0.01) and GSH-PX by 35% -38% (P <0.05) compared with lipid emulsion infusion group. Conclusions Increased FFA leads to increased oxidative stress in the body and may be one of the mechanisms leading to insulin resistance. After the application of sodium salicylate, oxidative stress is weakened and insulin resistance is improved. Therefore, sodium salicylate may play an important role in improving insulin resistance by reducing oxidative stress.