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目的:建立以高效液相色谱法测定肠炎清合剂中延胡索乙素含量的方法。方法:采用Hypersil ODS2 C_(18)柱色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-水-三乙胺(65:35:0.5),检测波长为280 nm,柱温为35℃;流速为1 ml·min~(-1)。结果:延胡索乙素在10～100μg·ml~(-1)范围内与峰面积积分值呈良好线性关系(r=0.999 9);平均加样回收率为96.81%,RSD=1.5%(n=6)。结论:本法快速、准确,重复性好,可用于肠炎清合剂的质量控制。
Objective: To establish a method for the determination of tetrahydropalmatine in Changyanqing Mixture by high performance liquid chromatography. METHODS: Hypersil ODS2 C_ (18) column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of methanol-water-triethylamine of 65:35:55. The detection wavelength was 280 nm and the column temperature was 35 ℃; flow rate of 1 ml · min ~ (-1). Results: Tetrahydropalmatine had a good linear relationship with the peak area (r = 0.999 9) in the range of 10-100 μg · ml -1. The average recoveries were 96.81%, RSD = 1.5% (n = 6). Conclusion: This method is rapid, accurate and reproducible. It can be used for the quality control of Changyin Qing Mixture.