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目的鼻咽癌是一种以放射治疗为主的头颈肿瘤,但部分鼻咽癌对放疗耐受。为了解鼻咽癌放疗耐受机制,本实验拟初步探讨人高分化鼻咽癌细胞株CNE1放疗后的蛋白质改变。方法利用固相pH梯度二维凝胶电泳,建立CNE1放疗前后总蛋白质2DE图谱,利用MALDI TOF MS及数据库搜索初步分析和鉴定部分放疗前后差异蛋白质点。结果高分子量酸性蛋白质在CNE1放疗后20min高表达,其中大多为热休克蛋白质HSPs(heatshockproteins,HSPs)。结论放射能诱导CNE1的热休克蛋白高表达,它们可能与鼻咽癌放疗耐受有关。
Purpose Nasopharyngeal carcinoma is a kind of head and neck cancer mainly radiotherapy, but some nasopharyngeal carcinoma is resistant to radiotherapy. In order to understand the mechanism of radiotherapy tolerance of nasopharyngeal carcinoma, this experiment intends to preliminary study the protein changes of human well-differentiated nasopharyngeal carcinoma cell line CNE1 after radiotherapy. Methods The 2DE maps of total protein before and after radiotherapy were established by two-dimensional gel electrophoresis with solid-phase pH gradient. The differential protein spots before and after radiotherapy were analyzed and identified by MALDI-TOF MS and database search. Results High molecular weight acidic proteins were highly expressed at 20 min after radiotherapy of CNE1, most of which were heat shock proteins (HSPs). Conclusion Radiation-induced high expression of heat shock proteins in CNE1 may be related to radiotherapy tolerance of nasopharyngeal carcinoma.