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目的:探讨凋亡抑制基因Survivin和抑癌基因PTEN在喉鳞状细胞癌(LSCC)中的表达及临床意义。方法:应用免疫组织化学Envision法检测71例LSCC标本中Survivin、PTEN蛋白的表达,并以10例声带息肉组织为对照。结果:Survivin蛋白在声带息肉中不表达;在71例LSCC中,53例(74.6%)表达阳性;Survivin阳性表达率与LSCC患者的年龄、性别、肿瘤大小均无相关性(均P>0.05),均与肿瘤发生部位、临床分期、病理分级、淋巴结转移、预后有关(均P<0.05)。PTEN蛋白在声带息肉中强表达(100%);在71例LSCC中,33例(46.5%)表达阳性;PTEN阳性表达率与LSCC患者的年龄、性别、肿瘤大小、发生部位均无相关性(均P>0.05),均与临床分期、病理分级、淋巴结转移、预后有关(均P<0.05)。在LSCC组织中Survivin与PTEN的表达呈负相关(P<0.05)。结论:Survivin、PTEN基因的异常表达与LSCC的发生、发展及预后有关,可作为评价LSCC生物学行为的重要参考指标。二者在LSCC的发生、发展中起协同作用。
Objective: To investigate the expression and clinical significance of Survivin and tumor suppressor gene PTEN in laryngeal squamous cell carcinoma (LSCC). Methods: The expressions of Survivin and PTEN protein in 71 cases of LSCC were detected by Envision immunohistochemistry. Ten cases of vocal cord polyp were used as control. Results: Survivin protein was not expressed in vocal cord polyps. In 71 LSCCs, 53 (74.6%) were positive for Survivin protein. There was no correlation between Survivin expression and age, sex and tumor size (all P> 0.05) , Were related to tumor site, clinical stage, pathological grade, lymph node metastasis and prognosis (all P <0.05). PTEN protein was strongly expressed in vocal cord polyp (100%). In 71 LSCCs, 33 (46.5%) were positive for PTEN protein. There was no correlation between PTEN expression and age, sex, tumor size and location All P> 0.05). All of them were related to clinical stage, pathological grade, lymph node metastasis and prognosis (all P <0.05). The expression of Survivin and PTEN was negatively correlated in LSCC (P <0.05). Conclusion: The abnormal expression of Survivin and PTEN gene is related to the occurrence, development and prognosis of LSCC, which may be used as an important reference index for evaluating the biological behavior of LSCC. Both play a synergistic role in the occurrence and development of LSCC.