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目的研究胸腺细胞对不同亚群胸腺基质细胞生长及功能的调节作用。方法胸腺基质细胞增殖以３Ｈ－ＴｄＲ掺入法测定，ＩＬ－７活性以促ＩＬ－７依赖株法检测，其ｍＲＮＡ表达以ＲＴ－ＰＣＲ法检测。结果胸腺细胞与ＭＴＳＣ４细胞分别以８０∶１，４０∶１或２０∶１的比例共育时，能明显抑制ＭＴＳＣ４细胞的增殖，而培养上清中ＩＬ－７活性无明显改变；当两种细胞的比例为１０∶１或５∶１时，则对ＭＴＳＣ４细胞的增殖无任何影响，而培养上清中的ＩＬ－７活性明显升高。ＲＴ－ＰＣＲ证实，与胸腺细胞共育的ＭＴＥＣ１和ＭＴＳＣ４细胞，ＩＬ－７ｍＲＮＡ水平明显提高。结论胸腺细胞能促进ＭＴＥＣ１和ＭＴＳＣ４细胞分泌ＩＬ－７，对不同亚群基质细胞的生长和功能有不同调节作用
Objective To study the regulatory effect of thymocytes on the growth and function of different subpopulations of thymus stromal cells. Methods Thymic stromal cell proliferation was measured by 3H-TdR incorporation assay. The activity of IL-7 was detected by IL-7-dependent assay. The mRNA expression of thymus was detected by RT-PCR. Results The proliferation of MTSC4 cells was significantly inhibited when the thymocytes were co-cultured with MTSC4 cells in the ratio of 80: 1, 40: 1 or 20: 1, while the activity of IL-7 in the culture supernatants was not significantly changed. When both cells 10: 1 or 5: 1 had no effect on the proliferation of MTSC4 cells, while the activity of IL-7 in the culture supernatant was significantly increased. RT-PCR confirmed that MTEC1 and MTSC4 cells co-cultured with thymocytes, IL-7 mRNA levels were significantly increased. Conclusion Thymocytes can promote the secretion of IL-7 by MTEC1 and MTSC4 cells, and have different regulatory effects on the growth and function of different subpopulations of stromal cells