本文采用双波长分光光度法测定蜂蜜普鲁卡因合剂中盐酸普鲁卡因及尼泊金乙酯的含量.盐酸普鲁卡因在3～15μg/ml范围内与吸收度呈良好的线性关系(r=1.0000),回收率为99.88%、RSD为0.23%;尼泊金乙酯在1.2～6.0μg/ml范围内与吸收度呈良好的线性关系(r=0.9999),回收率为99.85%,RSD为0.40%.该法操作简单,快速、准确.适应于医院对该制剂的质量控制. In this paper, dual-wavelength spectrophotometric determination of procaine hydrochloride and promethazine hydrochloride mixture of ethylparaben hydrochloride content of procaine hydrochloride in the range of 3 ~ 15μg / ml and absorbance showed a good linear relationship (r = 1.0000), the recovery was 99.88% and the RSD was 0.23%. The ethylparaben showed a good linear relationship with the absorbance (r = 0.9999) in the range of 1.2-6.0μg / ml and the recovery was 99.85% , RSD 0.40% .The method is simple, rapid and accurate.It is suitable for the quality control of the preparation in the hospital.