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目的:通过观察肉桂水提取物对重型颅脑损伤大鼠脑细胞凋亡、血清白细胞介素(interleukin,IL)-6的影响,探讨肉桂对重型颅脑损伤脑组织的保护作用。方法:54只SD大鼠随机分为三组,根据Feeney′s自由落体打击造模法复制动物模型。于伤后6h灌胃。灌胃后24h、72h、168h分别通过TUNEL法、ELISA法检测脑细胞凋亡、血清IL-6的含量。结果:(1)重型颅脑损伤大鼠受伤后血清IL-6明显升高,持续至168h。用肉桂水提取物干预后,在72h明显加重血清IL-6的升高,而在168h可以抑制血清IL-6的升高。(2)重型颅脑损伤大鼠在受伤后存在明显的脑细胞凋亡,这种凋亡一直持续至168h。用肉桂水提取物干预后,在72h明显加重这种凋亡,而在168h可以改善脑细胞凋亡。其作用与IL-6的含量相关。结论:肉桂水提取物对重型颅脑损伤早期用药会加剧炎症反应和细胞凋亡,三天后用药则呈现明显的保护作用。
OBJECTIVE: To observe the effect of cinnamon water extract on brain apoptosis and serum interleukin (IL) -6 in rats with severe craniocerebral injury and to explore the protective effect of cinnamon on brain injury in severe craniocerebral injury. Methods: Fifty-four SD rats were randomly divided into three groups and their animal models were reproduced according to Feeney’s free-fall modeling. Grouped 6h after injury. 24h, 72h, 168h after gavage, the apoptosis of brain cells and the content of serum IL-6 were detected by TUNEL and ELISA respectively. Results: (1) Serum IL-6 levels were significantly increased after severe traumatic brain injury in rats, lasting up to 168 hours. After intervention with cinnamon water extract, the increase of serum IL-6 was obviously increased at 72h, while the increase of serum IL-6 could be inhibited at 168h. (2) There was obvious apoptosis of brain cells in the injured rats after severe traumatic brain injury, which lasted until 168h. After intervention with cinnamon water extract, this apoptosis was significantly increased at 72h, and at 168h, apoptosis of brain cells was improved. Its role and IL-6 content. CONCLUSION: Cinnamon water extract can aggravate inflammatory response and apoptosis in the early stage of severe craniocerebral injury. Three days later, the drug showed obvious protective effect.