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目的采用支原体快速鉴定培养与药敏试验,观察呼吸道感染的患儿肺炎支原体的感染率和药敏情况。方法采集咽拭子标本做肺炎支原体快速鉴定培养与药敏试验。结果 1272例呼吸道感染患儿肺炎支原体快速鉴定培养中阳性449例,阳性率35.3%。支原体培养阳性的449例分别利用9种抗生素进行了药敏试验,支原体最敏感的为加替沙星99.3%、司帕沙星98.7%、左氧氟沙星95.6%。其次最敏感的是乙酰螺旋霉素95.6%、阿奇霉素92.4%;肺炎支原体耐药率最高的是克林霉素12.9%,其次是红霉素10.2%、罗红霉素9.6%、克拉霉素7.6%。结论肺炎支原体快速鉴定培养与药敏试验操作简单、快速、特异,标本易采集,直接药敏结果可指导临床合理用药,检测结果能满足临床需求,可为临床诊疗提供科学依据。
Objective To use mycoplasma rapid identification culture and drug sensitivity test to observe the infection rate and drug sensitivity of Mycoplasma pneumoniae in children with respiratory tract infection. Methods Throat swab specimens were collected for rapid identification of Mycoplasma pneumoniae and susceptibility testing. Results A total of 442 positive samples of Mycoplasma pneumoniae were detected in 1272 children with respiratory tract infection, the positive rate was 35.3%. Mycoplasma positive 449 cases were tested using nine antibiotics susceptibility test, the most sensitive mycoplasma 99.3% gatifloxacin, sparfloxacin 98.7%, 95.6% levofloxacin. The second most sensitive were acetylspiramycin 95.6% and azithromycin 92.4%. The highest rate of Mycoplasma pneumoniae was clindamycin 12.9%, followed by erythromycin 10.2%, roxithromycin 9.6%, clarithromycin 7.6 %. Conclusion Mycoplasma pneumoniae rapid identification of culture and susceptibility testing simple, rapid and specific, easy collection of specimens, direct drug susceptibility results can guide clinical rational use of drugs, test results to meet clinical needs, clinical diagnosis and treatment can provide a scientific basis.