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应用本教研室制备的兔抗AVP血清,我们建立了人及大鼠未经提取的血浆AVP的放射免疫测定法(RIA)。本法灵敏度为1.2pg/管,相当于血浆浓度为4pg/ml。回收率分别为112％(人血浆)和117％(鼠血浆)。加样量与测出量及与标准曲线之间有很好的平行关系。组内和多间变异系数(C.V.％)分别为.24％和23.04％。用本法测得4名正常人血浆AVP含量平均为5.2pg/ml,10只正常大鼠血浆AVP浓度为4.2±0.34pg/ml(X±SE)。’禁水36小时后,大鼠血浆AVP含量为34.3±9.7 pg/ml(X±SE)。本法能测定病理生理状态下AVP含量的变化,为某些疾病的诊断及研究提供一种有效的手段。
Using rabbit anti-AVP serum prepared in our laboratory, we established a radioimmunoassay (RIA) of human and rat plasma AVP without extraction. This method has a sensitivity of 1.2 pg / tube, which corresponds to a plasma concentration of 4 pg / ml. The recoveries were 112% (human plasma) and 117% (murine plasma), respectively. There is a good parallel relationship between the sample volume and the measured volume and the standard curve. Within the group and multiple coefficient of variation (C.V.%) were 24% and 23.04%. The plasma AVP levels in four normal individuals measured by this method were 5.2 pg / ml on average, and the plasma AVP concentrations in 10 normal rats were 4.2 ± 0.34 pg / ml (X ± SE). 36 hours after water forbidden, the plasma AVP content in rats was 34.3 ± 9.7 pg / ml (X ± SE). This method can determine the change of the content of AVP in the state of pathophysiology and provide an effective measure for the diagnosis and research of some diseases.