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目的:采用高效液相色谱法测定荡石片中绿原酸的含量。方法:以十八烷基硅烷键合硅胶为填充剂,以乙腈—0.5%磷酸溶液(11:89)为流动相,流速1.0ml·min-1,检测波长326nm。结果绿原酸进样量在0.40~3.20μg范围内与峰面积具有良好的线性关系(r=0.9999),精密度试验RSD为1.23%,重复性试验RSD为1.11%,平均回收率为99.78%,RSD为1.19%(n=9)。结论:本方法操作简便,精密度好,结果准确可靠,适用于荡石片中绿原酸含量的测定。
Objective: To determine the content of chlorogenic acid in rock fragments by high performance liquid chromatography. Methods: The octadecylsilane bonded silica gel was used as the filler, and acetonitrile-0.5% phosphoric acid solution (11:89) was used as the mobile phase. The flow rate was 1.0 ml · min-1 and the detection wavelength was 326 nm. Results There was a good linearity (r = 0.9999) for the injection of chlorogenic acid in the range of 0.40 ~ 3.20μg, the RSD of precision test was 1.23%, RSD of repeatability test was 1.11% and the average recovery was 99.78% , RSD was 1.19% (n = 9). Conclusion: The method is simple, accurate and accurate. The method is suitable for the determination of chlorogenic acid content in rock fragments.