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目的探讨乙肝疫苗无、弱应答与人类白细胞抗原的DRB1*0701基因位点(HLA-DRB1*0701)和环境因素之间的关系。方法选取1 342名接种乙肝疫苗后无、弱应答者128人作为病例组,在有应答者中按性别匹配随机抽取128人作为对照组;采用病例对照研究方法,对所有研究对象进行问卷调查,并应用PCR-序列特异引物(SSP)技术检测研究对象的HLA-DRB1*0701基因。采用SPSS 13.0软件对获取的研究数据进行单因素分析以及多因素Logistic回归分析。结果单因素分析发现,与乙肝疫苗接种无、弱应答相关的环境因素为年龄,OR=2.52,95%CI=1.51~4.21;体质指数(BMI),OR=2.38,95%CI=1.13~5.02;吸烟,OR=2.15,95%CI=1.08~4.28;饮茶,OR=0.32,95%CI=0.10~0.99。携带HLA-DRB1*0701基因者发生无、弱应答的风险为不携带此基因的4.83倍(95%CI=1.76~13.24);多因素Logistic回归分析最终进入模型的因素:年龄,OR=2.12,95%CI=1.24~3.63;BMI,OR=2.21,95%CI=1.04~4.72;HLA-DRB1*0701基因,OR=4.08,95%CI=1.45~11.46。结论乙肝疫苗接种无、弱应答的发生与多种因素有关。
Objective To explore the relationship between the non-response and non-response of hepatitis B vaccine and HLA-DRB1 * 0701 locus and environmental factors in human leukocyte antigen (HLA). Methods One hundred and seventy-two non-responders were enrolled in this study. One hundred and twenty-two non-responders were selected as the case group. 128 respondents were randomly selected as the control group by sex match. A case-control study was conducted to investigate all subjects, The HLA-DRB1 * 0701 gene was detected by PCR-SSP. Univariate analysis and multivariate logistic regression analysis were used to analyze the obtained data using SPSS 13.0 software. Results The univariate analysis showed that the environmental factors related to no or weak response to hepatitis B vaccination were age, OR = 2.52, 95% CI = 1.51-4.21, BMI, OR = 2.38, 95% CI = 1.13-5.02 ; Smoking, OR = 2.15, 95% CI = 1.08 ~ 4.28; drinking tea, OR = 0.32, 95% CI = 0.10 ~ 0.99. The risk of no-and-poor response to HLA-DRB1 * 0701 gene was 4.83-fold (95% CI = 1.76 to 13.24) in the absence of this gene. Multivariate Logistic regression analysis showed that the final entry into the model was age, OR = 2.12, 95% CI = 1.24-3.63; BMI, OR = 2.21, 95% CI = 1.04-4.72; HLA-DRB1 * 0701 gene, OR = 4.08, 95% CI = 1.45-11.46. Conclusion Hepatitis B vaccination without, the occurrence of weak response and a variety of factors.