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目的 研究钙通道拮抗剂对大鼠胰岛细胞分泌胰岛素的影响和机制。方法 用放免和荧光方法分别检测不同治疗血浓度的硝苯地平 (NIF)、维拉帕米 (VER)、地尔硫 (DIL)等药物对低糖和高糖刺激状态下胰岛细胞Ca2 + 含量和胰岛素分泌量的影响。结果 低糖状态组 :NIF、VER、DIL在 2 5、50、10 0 μg/L药物浓度下未对胰岛细胞内Ca2 + 含量和胰岛素分泌量产生影响 ,其结果与对照组相比差异无统计学意义 (P >0 0 5)。在高糖状态组 ,NIF、VER、DIL在 2 5μg/L药物浓度下不抑制胰岛素分泌 ,NIF在 50、10 0 μg/L浓度时 ,胰岛细胞内Ca2 + 浓度和胰岛素分泌量明显减少 ,与对照组相比差异有显著性 (P <0 0 5)并呈剂量相关 (P <0 0 1)。VER在 50 μg/L时胰岛素分泌有减少趋势 ,10 0 μg/L时明显减少 (P <0 0 5)。DIL在 10 0 μg/L时胰岛素分泌量减少与对照组相比差异具有显著性 (P <0 0 5)。结论 高浓度药理治疗量的NIF、VER、DIL具有抑制高糖作用下的胰岛细胞外钙内流和使胰岛素分泌减少的作用
Objective To study the effect and mechanism of calcium channel blockers on insulin secretion in pancreatic islets of rats. Methods Radioimmunoassay (RIA) and fluorescence were used to detect the contents of Ca2 + in islet cells under hypoglycemic and hyperglycemic conditions, such as nifedipine (NIF), verapamil (VER) and diltiazem (DIL) And the impact of insulin secretion. Results In the low glucose group, NIF, VER and DIL had no effect on intracellular Ca2 + content and insulin secretion at 2, 5, 50 and 10 μg / L drug concentrations. There was no significant difference between the two groups Significance (P> 0 0 5). In the high glucose group, NIF, VER and DIL did not inhibit insulin secretion at a concentration of 25μg / L. The intracellular Ca2 + concentration and insulin secretion were significantly decreased when NIF was at 50 and 10 μg / L, The difference between the two groups was significant (P <0 05) and dose-dependent (P 0 01). Insulin secretion decreased with VER at 50 μg / L, but decreased significantly at 100 μg / L (P <0.05). Compared with the control group, the decrease of insulin secretion of DIL at 100 μg / L was significant (P <0.05). Conclusion The high concentration of pharmacological treatment of NIF, VER, DIL can inhibit the extracellular extracellular calcium influx under the action of high glucose and reduce the role of insulin secretion