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目的:建立寿胎丸的高效液相色谱(HPLC)指纹图谱,为科学评价及有效控制寿胎丸质量提供可靠方法。方法:采用高效液相色谱法,选用Agilent HC-C18色谱柱,流动相为乙腈(A)-(0.05mol/L)KH2PO4-H3PO4缓冲溶液pH=3.0(B),线性洗脱梯度,检测波长为220nm;流速为1.0mL/min。结果:成功建立寿胎丸的HPLC指纹图谱,确立了36个共有峰,并且利用“中药色谱指纹图谱相似度评价软件”对相似度进行评价,相似度良好,达到实验标准。结论:该方法准确可靠,重复性好,为寿胎丸的质量控制提供科学准确的依据。
Objective: To establish HPLC fingerprint of ShouTai Wan and provide a reliable method for scientific evaluation and effective control of the quality of ShouTai Wan. Methods: The mobile phase consisted of acetonitrile (A) - (0.05mol / L) KH2PO4-H3PO4 buffer solution pH = 3.0 (B), linear elution gradient and detection wavelength 220 nm; flow rate 1.0 mL / min. Results: The HPLC fingerprints of ShouTaiWan were successfully established, 36 common peaks were established, and the similarities were evaluated by similarity evaluation software of TCM chromatographic fingerprinting. The similarity was good and reached the experimental standard. Conclusion: The method is accurate and reliable with good repeatability and provides a scientific and accurate basis for the quality control of ShouTai Wan.