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卫氏并殖吸虫在不适宜宿主体内以童虫形式长期滞留在肌肉组织内,难以发现,所以诊断常采用免疫学方法检测抗体。但由于童虫太小人们无法获得足够抗原,因而常用成虫抗原代替。本文评价了成虫抗原检测感染大鼠抗童虫抗体的价值,分析与鼠抗血清反应的成虫抗原特性以及抗原在虫体上的定位。实验动物家猫和Wistar大鼠每只分别经口感染20个和10个卫氏并殖吸虫囊蚴,并分别在感染后140天和70天从心脏取血,血清保存于-80℃,自感染38天起每天粪中查卵,最后解剖动物检出虫体。成虫抗原制作按Yokogawa(1956)法,用Yokogawa等(1983)ELISA技术检测血清抗体浓度,用SDSPAGE和免疫印渍技术分析与血清反应的抗
Paragonimus westermani in host unsuitable for long-term retention in the form of schistosomiasis muscle tissue, hard to find, so the diagnosis is often used immunological detection of antibodies. However, since the squirrels are too small, people can not get enough antigen, which is commonly used adult antigen instead. In this paper, we evaluated the value of adult antigen detection of anti-squirrel antibodies in infected rats, analyzed the characteristics of adult antigens reacted with mouse anti-serum and the localization of the antigens on the worms. Each of the experimental animals, domestic cats and Wistar rats, was orally infected with 20 and 10 Paragonimus westerhii metacercariae, respectively, and blood was taken from the heart at 140 days and 70 days after infection, respectively, and the serum was stored at -80 ° C 38 days of infection every day in the stool ovulation, the last anatomical animal detection of parasites. Adult antigen production Serum antibody concentrations were measured by the Yokogawa et al. (1983) ELISA technique according to the Yokogawa (1956) method. Anti-serum antibody levels were analyzed by SDSPAGE and immunoblotting