论文部分内容阅读
[目的]探讨正肝方对黄曲霉毒素B1(AFB1)诱发的大鼠癌前病变肝组织γ-谷氨酰转移酶(GGT)、谷胱甘肽S-转移酶-π(GST-P)阳性灶的影响。[方法]Wistar大鼠100只随机分为4组:模型组30只、正肝方小剂量预防(小剂量)组30只、正肝方大剂量预防(大剂量)组30只、正常大鼠对照(正常)组10只。除正常组外,先后用AFB1和2-乙酰氨基芴(2-AAF)处理各组大鼠造模。大、小剂量组在造模期间,将正肝方水剂(0.6 g/ml,0.3 g/ml)按10ml/kg分别灌胃;模型组用无菌蒸馏水10 ml/kg灌胃。8周后处死大鼠,肝组织取材。组织化学法做肝组织GGT染色、免疫组化法做肝组织GST-P染色,电脑图像分析系统测量GGT和GST-P阳性灶的数量、大小。[结果]大剂量组大鼠肝组织GGT阳性灶为(10.91±4.25)个/cm2,灶总面积为(2.94±1.52)mm2/cm2;GST-P阳性灶为(24.75±10.99)个/cm2,灶总面积为(4.80±2.75)mm2/cm2,均显著低于模型组(P<0.01)。[结论]正肝方能减少AFB1诱发的大鼠癌前病变肝组织GGT和GST-P阳性灶的数量和大小,对AFB1诱发的肝癌前病变具有一定的预防作用。
[Objective] To explore the effect of Zhengganfang on the hepatic γ-glutamyl transferase (GGT), glutathione S-transferase-π (GST-P) induced by aflatoxin B1 (AFB1) Positive foci of influence. [Methods] One hundred Wistar rats were randomly divided into four groups: model group 30, Zhenggan Fang small dose prevention group 30, Zhenggan Fang large dose prevention group 30 rats, normal rats Control (normal) group of 10. In addition to the normal group, rats in each group were treated with AFB1 and 2-acetylaminofluorene (2-AAF) successively. Large and small dose group in the modeling period, will Zhengganfang agent (0.6 g / ml, 0.3 g / ml) by 10ml / kg respectively; model group with sterile distilled water 10ml / kg gavage. After 8 weeks, the rats were sacrificed and the liver tissues were drawn. GGT staining of liver tissue was performed by histochemical method, and GST-P staining of liver tissue was performed by immunohistochemistry. The number and size of GGT and GST-P positive foci were measured by computer image analysis system. [Results] The positive rate of GGT positive foci was (10.91 ± 4.25) / cm2, the total foci area was (2.94 ± 1.52) mm2 / cm2 and the average GST-P positive foci was (24.75 ± 10.99) / cm2 (4.80 ± 2.75) mm2 / cm2, which were significantly lower than those in model group (P <0.01). [Conclusion] Zhenggan Decoction can reduce the quantity and size of GGT and GST-P positive foci in AFB1-induced rat precancerous lesion and have a preventive effect on AFB1-induced precancerous lesion.