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目的:探讨nm23、VEGF-C及其受体VEGFR-3在大肠癌中的表达和三者间的相互关系,及其在大肠癌淋巴结转移和肿瘤进展中的意义。方法:采用免疫组化SP法检测97例大肠癌组织和97例正常大肠组织中nm23、VEGF-C及VEGFR-3的表达。结果:1)A、B期大肠癌及无淋巴结转移的大肠癌组织中nm23的阳性表达率分别为68.9%、68.2%,C、D期及有淋巴结转移的大肠癌组织中nm23阳性表达率分别为30.6%、25.8%,A、B期高于C、D期,无淋巴结转移高于有淋巴结转移,差别有统计学意义(P<0.05);VEGF-C阳性表达率在C、D期及有淋巴结转移的大肠癌组织中分别为75.0%、77.4%,在A、B期及无淋巴结转移的大肠癌组织中分别为49.2%、50.0%,两者比较,差别有统计学意义(P<0.05);VEGFR-3在C、D期及有淋巴结转移的大肠癌中的阳性表达率分别为63.9%、67.7%,在A、B期及无淋巴结转移的大肠癌组织中分别为41.0%、40.9%,差别有统计学意义(P<0.05。2)nm23与分化程度、肠壁侵犯深度、有无淋巴结转移及Duckes分期有关(P<0.05),VEGF-C及VEGFR-3与分化程度、淋巴结转移及Duckes分期有关(P<0.05)。3)nm23在正常大肠组织和大肠癌组织中的阳性表达率分别为83.5%、54.6%,差别有统计学意义(P<0.05),VEGF-C在正常大肠组织和大肠癌组织中的阳性表达率分别为29.9%、58.8%,差别有统计学意义(P<0.05),VEGFR-3在正常大肠组织和大肠癌组织中的阳性表达率分别为21.6%、49.5%,两者比较,差别有统计学意义(P<0.05)。结论:nm23与VEGF-C和VEGFR-3在大肠癌中的表达呈负相关关系,nm23基因对大肠癌有抑制作用,VEGF-C和VEGFR-3与大肠癌的侵袭和转移密切相关,三者的联合检测可作为评价大肠癌病情、推测预后及指导治疗的重要参考指标。
OBJECTIVE: To investigate the expression of nm23, VEGF-C and its receptor VEGFR-3 in colorectal cancer and the relationship among them, and their significance in lymph node metastasis and tumor progression in colorectal cancer. Methods: The expressions of nm23, VEGF-C and VEGFR-3 in 97 cases of colorectal carcinoma and 97 cases of normal colorectal tissues were detected by immunohistochemical SP method. Results: The positive expression rates of nm23 in colorectal cancer A and B and colorectal cancer without lymph node metastasis were 68.9%, 68.2% respectively. The positive rates of nm23 in C, D and colorectal cancer with lymph node metastasis were (P <0.05). The positive rates of VEGF-C in C and D stages were significantly higher than those in C and D stages (P <0.05) Colorectal cancer with lymph node metastasis was 75.0% and 77.4%, respectively, which were 49.2% and 50.0% respectively in stage A and B colorectal cancer without lymph node metastasis, the difference was statistically significant (P < 0.05). The positive rates of VEGFR-3 in C, D and colorectal carcinomas with lymph node metastasis were 63.9% and 67.7%, respectively, which were 41.0% in stage A, B and colorectal cancer without lymph node metastasis respectively, 40.9%, the difference was statistically significant (P <0.05.2) nm23 with the degree of differentiation, invasion depth of the intestinal wall, with or without lymph node metastasis and Duckes staging (P <0.05), VEGF-C and VEGFR- Lymph node metastasis and Duckes staging (P <0.05). 3) The positive rates of nm23 in normal colorectal and colorectal cancer were 83.5% and 54.6%, respectively, with statistical significance (P <0.05). The positive expression of VEGF-C in normal colorectal and colorectal cancer tissues The rates of positive expression of VEGFR-3 in normal colorectal cancer and colorectal cancer tissues were 21.6% and 49.5%, respectively, with a difference of 29.9% and 58.8%, respectively (P <0.05) Statistical significance (P <0.05). CONCLUSIONS: There is a negative correlation between the expression of nm23 and VEGF-C and VEGFR-3 in colorectal cancer. The nm23 gene has an inhibitory effect on colorectal cancer. VEGF-C and VEGFR-3 are closely related to the invasion and metastasis of colorectal cancer. The combined detection of colorectal cancer can be used as a condition to assess the prognosis and guide treatment of important reference index.