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目的探讨麻疹野病毒的病原学特征，为ＷＨＯ麻疹病毒参考资料库（ｄａｔａｂａｎｋ）提供国内样品株，填补我省麻疹抗原分离的空白。方法采用组织培养法，选用敏感细胞分离抗原，并用中和试验法进行野毒株的鉴定。结果在Ｂ９５－ａ细胞上共分离出１０株麻疹野病毒，其中７份咽拭液同时用Ｖｅｒｏ细胞分离未获成功，但将１０株分离株转种至Ｖｅｒｏ细胞上时，有４株出现麻疹的融合性细胞病变。１∶１０稀释的麻疹单克隆抗体（１２Ｅ）能中和所有分离株，在同期收集的９例麻疹患者血清中，４例麻疹ＩｇＭ抗体阳性，但在１例麻疹ＩｇＧ抗体滴度达１∶６４００至１∶≥１０２４００的患者中，也分离到了麻疹病毒。结论在湖南境内分离的１０株麻疹野毒中，９４—４７株为目前国际上最大程度的麻疹变异株，具有一定抗体水平的儿童仍受到９０年代野毒的攻击而形成临床感染。
Objective To explore the etiological characteristics of wild measles virus and to provide domestic sample strains to the databank of WHO measles virus to fill the blank of measles antigen isolation in our province. Methods Tissue culture method was used to select antigens by using sensitive cells. The neutralization test was used to identify the wild strains. Results Ten wild measles virus strains were isolated from B95-a cells. Seven of the pharyngeal pharyngeal swabs were not successfully isolated with Vero cells at the same time. When 10 isolates were transplanted onto Vero cells, 4 measles Of confluent cytopathic. The measles monoclonal antibody (12E), diluted 1:10, neutralized all isolates. Of the 9 measles patient sera collected during the same period, 4 measles IgM antibodies were positive, but 1 measles IgG antibody titer reached 1: 6400 To 1:> 102,400 patients, the measles virus was also isolated. Conclusion Among the 10 measles wild isolates isolated in Hunan Province, 94-47 strains are the most measles variant currently in the world. Children with a certain antibody level are still under the attack of wild poison in the 1990s to form a clinical infection.