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目的:观察异鼠李素对H2O2损伤的CRL-1730细胞的活性、凋亡率以及细胞周期的影响。方法:用55,27.5,13.75 mg.L-13种质量浓度的异鼠李素与培养的CRL-1730细胞置于37℃,5%CO2饱和湿度培养箱中共同孵育24 h,再用H2 O2氧化损伤4 h后,四甲基偶氮唑蓝(MTT)比色法检测异鼠李素对H2 O2损伤的细胞活性的影响,流式细胞仪测定异鼠李素对H2O2损伤的细胞周期及细胞凋亡的影响。结果:异鼠李素能够剂量依赖性增强CRL-1730细胞活性,与模型组吸光度(A)0.459比较,高剂量组A 0.503,升高了0.044,有显著性差异(P<0.01),中剂量组A 0.48,升高了0.027,差异有统计学意义(P<0.05),低剂量组无明显影响。异鼠李素能够剂量依赖性减少受损细胞的凋亡,降低早期凋亡率,与模型组凋亡率77.78%相比,高剂量组69.28%和中剂量组72.50%差异均有统计学意义(P<0.05)。异鼠李素能抑制H2 O2引起的CRL-1730细胞减少,表现在使G0/G1期细胞比例减少,模型组G0/G1期细胞比例为82.23%,异鼠李素高,中,低剂量组该值分别为69.43%,67.05%,69.56%,均有显著性差异;S期和C2/M期细胞比率增加,模型组S期和C2/M期细胞比率为11.77%和1.91%,异鼠李素高剂量组为23.39%和7.18%,中剂量组为29.73%和3.23%,低剂量组为27.42%和3.01%。差异均有统计学意义(P<0.05或P<0.01)。结论:异鼠李素对H2O2损伤的CRL-1730细胞具有保护作用。
Objective: To investigate the effect of isorhamnetin on the activity of H2O2-induced CRL-1730 cells, the apoptosis rate and cell cycle. Methods: Isorhamnetin (55, 27.5, 13.75 mg.L-13) and cultured CRL-1730 cells were incubated in a humidified incubator at 37 ° C and 5% CO2 for 24 h, then incubated with H2O2 After oxidative injury for 4 h, MTT assay was used to determine the effect of isorhamnetin on H2O2-induced cell injury. Flow cytometry was used to determine the cell cycle of isorhamnetin- Effect of apoptosis. Results: Isorhamnetin increased CRL-1730 cell activity in a dose-dependent manner. Compared with the 0.459 absorbance of model group (A), it increased 0.050 in high dose group (P <0.01) Group A 0.48, an increase of 0.027, the difference was statistically significant (P <0.05), low-dose group had no significant effect. Isorhamnetin can reduce the apoptosis of injured cells in a dose-dependent manner and reduce the rate of early apoptosis. Compared with the model group, the apoptosis rate of 77.78%, the high dose group 69.28% and the middle dose group 72.50% difference was statistically significant (P <0.05). Isorhamnetin could inhibit the decrease of CRL-1730 cells induced by H2O2, which showed that the proportion of cells in G0 / G1 phase decreased, the proportion of cells in G0 / G1 phase of model group was 82.23%, the values of isorhamnetin, medium and low dose groups were 69.43%, 67.05% and 69.56%, respectively. The percentage of cells in S phase and C2 / M phase increased, the percentage of cells in S phase and C2 / M phase in model group was 11.77% and 1.91% 23.39% and 7.18%, 29.73% and 3.23% in the middle dose group and 27.42% and 3.01% in the low dose group. The differences were statistically significant (P <0.05 or P <0.01). CONCLUSION: Isorhamnetin has a protective effect on CRL-1730 cells damaged by H2O2.