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红叶李(Prunus cerasifera)是重要的观叶植物,此树整个生长季节为紫红色,其花为粉红色,果呈球形.宜于建筑物大门前、庭园路旁或草坪角隅处栽植.若背景选择适当,它的色彩美更能显示出来.传统的繁殖方法主要是用桃、李、梅或山桃砧木进行嫁接.如果采用组织培养法通过试管增殖,可在短期内获得大量移栽小苗.材料和方法(一)培养材料的制备春季,当芽开始萌动时,取红叶李的嫩茎放入75%的酒精中2—3秒后立即倒出酒精,再用无菌水冲洗一次.取出嫩茎放入漂白粉饱和溶液中经25—30分钟灭菌后,倒出漂白粉溶液,再用无菌水冲洗3—5次.这一过程需在无菌室内或超净工作台上进行.将灭过菌的嫩茎,剪成茎段(长度为1.0—1.5厘米)每个茎段上至少带有一个腋芽.以此作为培养材料.
Prunus cerasifera is an important foliage plant that has a purple-red color throughout its growing season, pink-colored flowers with spherical globes, and is suitable for planting in front of the building, on the paved roadside or in lawn corners. If the background is selected properly, its color beauty is more displayed.Traditional breeding methods are mainly used peach, plum or plum rootstock grafting.If the tissue culture method through the tube proliferation, in a short period of time to obtain a large number of transplanting seedlings MATERIALS AND METHODS A. Preparation of Culture Materials In the spring, when buds begin to germinate, pour the tender stems of the leaves into 75% alcohol for 2-3 seconds and then pour out the alcohol and rinse once with sterile water. Remove the tender stem into the bleach saturated solution after 25-30 minutes after sterilization, pour bleach solution, and then washed 3-5 times with sterile water.This process needs to be carried out in sterile indoor or clean bench. The sterilized young stems are cut into stem segments (1.0-1.5 cm in length) with at least one axillary bud on each stem, which is used as a culture material.