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目的：研究新基因RA28的读码框及其蛋白的性质，并检测其在不同肿瘤细胞系中的表达情况，探讨其与肿瘤发生的关系。方法：采用原核表达系统，获得高效表达，产物经金属螯合亲和层析纯化；Westernblot分析RA28蛋白在不同细胞系中的表达情况。此外，借助计算机辅助分析。结果：RA28蛋白的分子量为12KDa，等电点为7.1；RA28蛋白在所检测的细胞系中均表达,未见细胞表达的特异性；同源序列比较表明，RA28与Mat-8(Mammarytumor8Kda)有很高的同源性。结论：RA28可能参与细胞信号传导，并与肿瘤发生相关。
Objective: To study the reading frame of new gene RA28 and the nature of its protein, and to detect the expression of RA28 in different tumor cell lines and to explore its relationship with tumorigenesis. Methods: Prokaryotic expression system was used to obtain high expression. The product was purified by metal chelate affinity chromatography. The expression of RA28 protein in different cell lines was analyzed by Western blot. In addition, with computer-aided analysis. Results: The molecular weight of RA28 protein was 12 KDa and the isoelectric point was 7.1. The expression of RA28 protein was detected in all the cell lines tested. No specific expression of RA28 protein was found. The homology comparison of RA28 with Mat-8 (Mammary tumor 8 Kda) High homology. Conclusion: RA28 may be involved in cell signaling and related to tumorigenesis.