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目的:建立一种毛细管电泳电致化学发光测定琥乙红霉素的新分析方法。方法:利用铕离子掺杂普鲁士蓝化学修饰铂电极对联吡啶钌的电催化氧化作用可显著增强电致化学发光法检测琥乙红霉素的灵敏度,据此建立了毛细管电泳-化学修饰电极电致化学发光检测琥乙红霉素的分析新方法。结果:在优化的实验条件下,发光强度与琥乙红霉素的浓度在1～100μg.mL-1(r=0.9993)之间呈良好线性关系,检出限为0.25μg.mL-1(S/N=3)。对含10.0μg.mL-1琥乙红霉素标样连续测定5次,发光强度和迁移时间的RSD分别为2.2%,0.81%。结论:本法具有分离度好,灵敏高,分析试样无需复杂处理可直接进行测定等优点,可对药物制剂和合成人工尿样中琥乙红霉素含量进行简便、快速的测定。
Objective: To establish a new capillary electrophoresis chemiluminescence determination of erythromycin ethylsuccinate new analytical methods. Methods: The electrocatalytic oxidation of bipyridyl ruthenium with platinum-doped Prussian blue modified platinum electrode can significantly enhance the sensitivity of the electrochemiluminescence method for the determination of erythromycin ethylsuccinate. Based on this, the capillary electrophoresis-chemically modified electrode Chemiluminescence detection of erythromycin ethylsuccinate new method of analysis. Results: Under the optimized experimental conditions, there was a good linear relationship between the luminescence intensity and the concentration of erythromycin ethylsuccinate at 1 ~ 100μg.mL-1 (r = 0.9993) with the detection limit of 0.25μg.mL-1 ( S / N = 3). The RSDs of luminescence intensity and migration time of the standard samples containing 10.0μg.mL-1 erythromycin ethylsuccinate were measured continuously for 5 times respectively, and the RSDs were 2.2% and 0.81% respectively. Conclusion: The method has the advantages of good resolution, high sensitivity and direct analysis of samples without complicated treatment. The method can be used to determine the content of erythromycin ethylsuccinate in pharmaceutical preparations and synthetic artificial urine easily and rapidly.