论文部分内容阅读
目的:探讨微波治疗后肝癌组织中survivin的表达及其意义。方法:对不能或不宜手术治疗的原发性肝癌8例,肝转移癌16例,共24例,40个癌灶,行超声引导下微波治疗。采用RT-PCR检测肝癌组织中微波术前以及术后2周survivin mRNA的表达。结果:1个月后CT检查,显示31个病灶被完全灭活(77.5%,31/40),不完全坏死或部分坏死(癌瘤残存或者复发)9个(22.5%)。术前以及术后2周采用RT-PCR检测24例肝癌组织中survivin mRNA表达,其中有17例survivin mRNA表达下降与术前比较,有统计学差异(P<0.05)。治疗前后肝癌组织survivin mRNA表达无差异者(29.2%,7/24)与癌瘤结节残存或者复发(22.5%,9/40)一致(P>0.05)。结论:微波凝固是一种有效、安全、简便治疗肝癌的方法。肝癌组织的survivin mRNA的表达可以作为及时评估疗效的指标之一。
Objective: To investigate the expression of survivin in hepatocellular carcinoma after microwave treatment and its significance. Methods: 8 cases of primary hepatic carcinoma, 16 cases of hepatic metastases, 24 cases, 40 lesions were treated with microwave or not guided by ultrasound. The expression of survivin mRNA in liver cancer tissue before and after 2 weeks of operation was detected by RT-PCR. Results: One month after the CT examination, 31 lesions were completely inactivated (77.5%, 31/40), incomplete necrosis or partial necrosis (residual or relapsed cancer), 9 (22.5%). The expression of survivin mRNA in 24 HCC tissues was detected by RT-PCR two weeks after operation. The expression of survivin mRNA in 17 cases was significantly lower than that before operation (P <0.05). Before and after treatment, there was no difference in the expression of survivin mRNA between the two groups (29.2%, 7/24) and the recurrence (22.5%, 9/40) of tumor nodules (P> 0.05). Conclusion: Microwave coagulation is an effective, safe and simple method for the treatment of liver cancer. The expression of survivin mRNA in hepatocellular carcinoma can be used as an index to evaluate the curative effect in time.