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[目的]建立标准的Nestin-LacZ转基因小鼠脊髓损伤动物模型及评价体系。[方法]采用NestinLac Z转基因小鼠18只,随机分为三组,实验组2个,对照组1个。2个实验组均采用自行研制的啮齿类动物脊髓损伤模型打击器,质量为10 g的打击棒,A实验组用接触直径为2.0 mm的打击棒杆头压迫,压迫时间为3 min(A组,6例);B实验组按照实验设定的高度10.0 mm实施打击(B组,6例);对照组仅行椎板切除,不打击(C组,6例)。分别于术前,术后第1、2、3、4、5、6周进行转基因小鼠BBB运动评分,取出脊髓组织后,行脊髓组织HE染色。[结果]Nestin-LacZ转基因小鼠不同的脊髓损伤模型在各个时间点上BBB评分差异有统计学意义(P<0.05),A组在脊髓损伤造模术后2周,后肢功能开始恢复,而B组至术后6周均无后肢功能恢复,对照组无功能障碍。[结论]啮齿类动物脊髓损伤模型打击器采用持续慢性压迫脊髓可成功建立Nestin-Lac Z转基因小鼠稳定、理想的急性脊髓损伤模型。
[Objective] To establish a standard animal model and evaluation system of spinal cord injury in Nestin-LacZ transgenic mice. [Method] Eighteen NestinLac Z transgenic mice were randomly divided into three groups, two in experimental group and one in control group. Both experimental groups were self-developed rodent spinal cord injury model hitter with a mass of 10 g. The experimental group A was pressured with a contact head 2.0 mm in diameter for 3 min (Group A , 6 cases). The experimental group B was hit at a height of 10.0 mm (group B, 6 cases). The control group was treated with laminectomy alone (group C, 6 cases). The BBB motor score of the transgenic mice were respectively preoperatively, 1, 2, 3, 4, 5, and 6 weeks after operation. Spinal cord tissue was removed for HE staining. [Result] The BBB scores of Nestin-LacZ transgenic mice at different time points were significantly different (P <0.05). The function of hindlimb began to recover in group A at 2 weeks after SCI No functional recovery of hindlimb was found in group B and 6 weeks after operation, while there was no dysfunction in control group. [Conclusion] The stable and ideal model of acute spinal cord injury in Nestin-Lac Z transgenic mice can be successfully established by sustained chronic compression of spinal cord in rodent spinal cord injury model.