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利用ＲＴＰＣＲ技术，自人的脾脏单核细胞ｍＲＮＡ扩增出人粒细胞集落刺激因子（ｈＧＣＳＦ）结构基因，ＤＮＡ序列分析表明与天然ｈＧＣＳＦ一致。将其克隆于表达载体ｐＪＧＷ１中，在大肠杆菌中进行诱导表达研究。结果表明：ｈＧＣＳＦ重组蛋白表达率在２５％以上，并具有与天然ｈＧＣＳＦ一致的生物学活性。
Using RT-PCR technology, human granulocyte colony stimulating factor (hG-CSF) structural gene was amplified from mRNA of human spleen mononuclear cells, DNA sequence analysis showed that it was consistent with natural hG-CSF. It was cloned in the expression vector pJGW1 and induced in Escherichia coli. The results showed that: hG CSF recombinant protein expression rate of 25% or more, and has the same with the natural hG CSF biological activity.