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目的 观察培养的大鼠胃平滑肌细胞中是否能表达GnRH受体 ,为进一步研究GnRH对胃平滑肌细胞的功能提供形态学依据。 方法 采用免疫组织化学SABC和原位杂交方法。 结果 培养的大鼠胃平滑肌细胞呈GnRH受体免疫反应阳性 ,阳性物质分布于细胞浆和细胞膜上 ,细胞核呈阴性反应。培养的胃平滑肌细胞同样含有GnRH受体mRNA杂交信号 ,信号物质亦分布于细胞浆内 ,细胞核呈阴性反应。 结论 胃平滑肌细胞能自身表达GnRH受体 ,它可能也是GnRH的靶细胞
Objective To observe whether GnRH receptor can be expressed in cultured rat gastric smooth muscle cells and to provide a morphological basis for further study on the function of GnRH in gastric smooth muscle cells. Methods Immunohistochemical SABC and in situ hybridization methods. Results The cultured rat gastric smooth muscle cells showed positive GnRH receptor immunoreactivity. The positive substances distributed in the cytoplasm and the cell membrane, and the nucleus was negative. Cultured gastric smooth muscle cells also contain GnRH receptor mRNA hybridization signals, signal substances are also distributed in the cytoplasm, the nucleus was negative. Conclusion Gastric smooth muscle cells express GnRH receptor by themselves and may also be target cells of GnRH