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[目的]探讨雌激素受体β(ERβ)在大肠癌发生发展过程中表达及其临床意义。[方法]收集正常人大肠黏膜、FAP腺瘤、FAP腺瘤癌变、腺瘤、腺瘤癌变和散发性大肠癌标本共162例,应用浙江大学附属第二医院自行研制的ZM-1型组织芯片制备仪制作石蜡组织芯片,行免疫组织化学染色。[结果]散发性大肠癌组ERβ阳性表达率(81.25%)明显高于正常大肠黏膜组(44.44%)(P=0.017);FAP腺瘤癌变组(47.62%)和腺瘤癌变组(38.89%)的ERβ阳性率分别低于FAP腺瘤组(80.00%)和腺瘤组(80.00%)(P=0.005,P=0.033);且FAP腺瘤癌变组和腺瘤癌变组的ERβ阳性率低于散发性大肠癌组(P<0.05);FAP腺瘤组与腺瘤组的ERβ阳性率却均高于正常大肠黏膜组(P<0.05)。[结论]ERβ在散发性大肠癌组织中的高表达提示ERβ可能是一个散发性大肠癌临床诊断的鉴别标志。FAP腺瘤和腺瘤癌变过程所涉及的分子机制可能与散发性大肠癌变分子机制不同。
[Objective] To investigate the expression and clinical significance of estrogen receptor β (ERβ) in the development of colorectal cancer. [Methods] A total of 162 specimens of colorectal mucosa, FAP adenoma, FAP adenoma, adenoma, adenoma and sporadic colorectal cancer were collected. ZM-1 tissue microarray developed by the Second Affiliated Hospital of Zhejiang University Preparation instrument production paraffin tissue chip, immunohistochemical staining. [Results] The positive expression rate of ERβ in sporadic colorectal cancer was significantly higher than that in normal colorectal mucosa (81.25% vs 44.44%, P = 0.017). The positive rates of ERβ in adenoma (47.62%) and adenoma (38.89% ) Were lower than that of FAP adenoma group (80.00%) and adenoma group (80.00%) (P = 0.005, P = 0.033). The positive rates of ERβ in FAP adenocarcinoma group and adenoma carcinogenesis group were lower (P <0.05). The positive rate of ERβ in FAP adenoma group and adenoma group was higher than that in normal colorectal mucosa group (P <0.05). [Conclusion] The high expression of ERβ in sporadic colorectal cancer suggests that ERβ may be a differential marker for the clinical diagnosis of sporadic colorectal cancer. The molecular mechanisms involved in the carcinogenesis of FAP adenomas and adenomas may be different from those of sporadic colorectal carcinomas.