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采用电化学聚合技术,在掺杂的导电聚吡咯薄膜修饰过的铅笔芯电极上,吸附葡萄糖氧化酶制备葡萄糖生物传感器。首先在含有0.1 mol/L吡咯和0.01 mol/L HCl的溶液中,于0.7 V恒电位下吡咯单体氧化聚合,在铅笔芯电极表面形成聚吡咯薄膜;然后将葡萄糖氧化酶吸附在修饰过的电极上制备出葡萄糖氧化酶-聚吡咯-铅笔芯电极电流型生物传感器。实验考察了吡咯聚合时间、聚合温度、葡萄糖氧化酶吸附量、检测电压以及干扰物对传感器性能的影响。实验结果表明,在优化条件下,传感器的灵敏度为17.78μA/mmol/L,线性范围0.8 mmol/L,线性相关度R=0.9918,响应时间小于16 s,检测下限为18.75μmol/L,有较强的抗干扰能力。
Adopting electrochemical polymerization technology, glucose biosensor was prepared by adsorbing glucose oxidase on the pencil core electrode doped with conductive polypyrrole film. Pyrrole monomer was first oxidized and polymerized at a constant potential of 0.7 V in a solution containing 0.1 mol / L pyrrole and 0.01 mol / L HCl to form a polypyrrole film on the surface of the pencil core electrode. Then, glucose oxidase was adsorbed on the modified Electrode prepared glucose oxidase - polypyrrole - pencil core electrode current biosensor. The effect of pyrrole polymerization time, polymerization temperature, glucose oxidase adsorption, detection voltage and interferences on the performance of the sensor was investigated experimentally. The experimental results show that under the optimal conditions, the sensitivity of the sensor is 17.78μA / mmol / L, the linear range is 0.8 mmol / L, the linear correlation is 0.9918, the response time is less than 16s, the detection limit is 18.75μmol / L, Strong anti-interference ability.