建立美斯替仑磷脂复合物复合率的间接测定方法。采用紫外分光光度法测定未参与复合的美斯替仑的量,由此计算复合率。结果表明,美斯替仑在269nm处有最大吸收,浓度在10.02—40.09μg·mL~(-1)时与吸光度呈良好的线性关系,回归方程为:A=9.0176C+0.0065,r=0.9999(n=3);平均回收率为99.54%,RSD为0.66%。本法操作简便易行,具有良好的重现性和精密度。 Indirect determination of recombination rate of memantinol phospholipid complex was established. UV spectrophotometry was used to determine the amount of metstigmine that was not involved in the complexation, thereby calculating the recombination rate. The results showed that there was a good linear relationship between absorbance and absorbance at the concentration of 10.02-40.09 μg · mL -1 at the maximum absorption of 263 nm. The regression equation was: A = 9.0176C + 0.0065, r = 0.9999 (n = 3). The average recovery was 99.54% with a RSD of 0.66%. This method is easy to operate, with good reproducibility and precision.