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建立了HPLC法检测酞丁安含量和色谱纯度。采用ODS-3色谱柱,流动相为乙腈-0．75％甲酸溶液(30:70),检测波长280nm与347nm。酞丁安在0．5-400μg／ml范围内线性关系良好(r=0．9999)。检测限为1．0ng。
A HPLC method was developed for the determination of the content and the chromatographic purity of ODS-3 was used as the mobile phase. The mobile phase was acetonitrile-0.75% formic acid solution (30:70). The detection wavelength was at 280 nm and 347 nm. Phthalidin had a good linear relationship in the range of 0.5-400 μg / ml (r = 0.9999). The detection limit is 1.0ng.