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杏仁体中的多巴胺(DA)和γ -氨基丁酸(GABA)递质系统均参与精神分裂症的病理过程,临床上一般用多巴胺II型受体(D2)阻断剂予以治疗。然而,目前尚不清楚GABA与D2受体是否共存,也不清楚DA能神经末梢与GABA能神经元之间的联系方式。本实验用共聚焦激光扫描显微镜(CLSM)和免疫电镜(IEM)研究了杏仁体关键性核团基底外侧核中GABA与D2受体的共存关系以及DA神经能末梢与GABA能神经元之间的突触关系。CLSM显示由谷氨酸脱羧酶(GAD)标记的GABA能神经元全部对D2受体呈免疫阳性反应,表明GABA能神经元含有D2受体。IEM显示,在 980个DA能神经末梢形成的突触中,45%的突触是由DA免疫反应阳性神经末梢直接(36% )或间接(9% )与GAD免疫反应阳性神经元的树突形成,另 55%是由DA免疫反应阳性神经末梢与未标记的神经元成分形成。DA GABA的直接性突触进而可区分为单突触 (16% )、汇聚突触 (14% )及轴 轴突触(6% )。而DA- GABA的间接性突触是个突触复合体。在该复合体中,DA免疫反应阳性末梢在一个未标记的末梢上形成对称性突触,而该未标记末梢又与GAD免疫反应阳性树突形成非对称性突触。在DA与未标记神经元成分之间的突触中,AD免疫反应阳性末梢分别与未标记胞体(4% )、树突(42% )及轴突末梢(9% )形成突触。所有DA突触无一例外均为?
Both amygdala dopamine (DA) and γ - aminobutyric acid (GABA) neurotransmitter systems are involved in the pathogenesis of schizophrenia and are generally treated clinically with dopamine type 2 receptor (D2) blockers. However, it is unclear whether GABA coexists with D2 receptors or how the DA nerve endings interact with GABAergic neurons. In this study, the confocal laser scanning microscopy (CLSM) and immunoelectron microscopy (IEM) were used to investigate the coexistence of GABA and D2 receptors in the basolateral nucleus of the amygdala and the relationship between DA nerve endings and GABAergic neurons Synaptic relationship. CLSM showed that GABAergic neurons labeled with glutamate decarboxylase (GAD) all immunoreactively reacted with D2 receptors, indicating that GABAergic neurons contain D2 receptors. IEM showed that of the synapses formed by 980 DA nerve endings, 45% of the synapses were dendrites directly (36%) or indirectly (9%) with GAD-immunoreactive neurons by DA immunoreactive positive nerve endings The other 55% is formed by DA immunoreactive positive nerve endings with unlabeled neuronal components. Synaptic synapses of DA GABA are in turn differentiated into monosynapses (16%), convergent synapses (14%) and axonal synapses (6%). Indirect synapses of DA-GABA are synaptic complexes. In this complex, DA immunoreactive positives form symmetrical synapses on one unlabeled tip that, in turn, form asymmetric synapses with the GAD immunoreactive positive dendrites. In the synapses between DA and unlabeled neuronal components, AD immunoreactive positive synapses formed unnamed soma (4%), dendrites (42%), and axon tips (9%), respectively. All DA synapses without exception are?