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目的:探讨Toll样受体2(TLR2)在喉鳞状细胞癌组织和癌旁组织中的表达及意义。方法:RT-PCR方法检测TLR2mRNA,Western-blot方法检测TLR2蛋白在22例喉鳞状细胞癌患者肿瘤组织及癌旁组织中的表达情况,并以声带息肉为对照。结果:RT-PCR及Western-blot检测结果表明,TLR2mRNA在癌及癌旁组织中均有表达,而在对照组织中几乎不表达。其在癌组织中的表达强度较在癌旁组织明显增高(t=4.279,P<0.01);在癌组织中的表达随肿瘤临床分期增高而增强(F=5.496,P<0.05);其表达强度与淋巴结是否转移(N分期)有关(F=4.271,P<0.05)。TLR2蛋白的表达水平与mRNA相一致。结论:TLR2mRNA和蛋白在喉鳞状细胞癌组织中的表达显著高于癌旁组织,随临床分期增高而表达增强,其表达水平与淋巴结转移有关。由此可见,TLR2可能通过多种机制参与喉癌的发生、发展。
Objective: To investigate the expression and significance of Toll-like receptor 2 (TLR2) in laryngeal squamous cell carcinoma and adjacent normal tissues. Methods: TLR2 mRNA was detected by RT-PCR. The expression of TLR2 protein was detected by Western-blot in 22 cases of laryngeal squamous cell carcinoma with vocal cord polyps. Results: The results of RT-PCR and Western-blot showed that TLR2 mRNA was expressed in both cancer and paracancerous tissues, but not in control tissues. The expression level in cancer tissues was significantly higher than that in paracancer tissues (t = 4.279, P <0.01). The expression in cancer tissues was enhanced with the clinical stage (F = 5.496, P <0.05) Intensity and lymph node metastasis (N staging) (F = 4.271, P <0.05). TLR2 protein expression level consistent with the mRNA. CONCLUSION: The expression of TLR2 mRNA and protein in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissues, and increased with the clinical stage. The expression of TLR2 mRNA and protein was correlated with the lymph node metastasis. Thus, TLR2 may participate in the occurrence and development of laryngeal carcinoma through a variety of mechanisms.