1986年将受粉后3周的刚果野芝麻和受粉后2周的广东崖州小黑籽的蒴果在70%的乙醇中浸泡半分钟,然后用0.1%HgCl_2表面灭菌12分钟。在超净工作台上剥去种皮取出胚,分别接种于培养基:(1)L.S.M+0.2mg/L6-BA+蔗糖2%,pH5.6,(2)L.S.M+0.2mg/L6—BA+蔗糖2%+水解乳蛋白600mg/L,pH5.6。在25—29℃,每天光照12小 In 1986, Congo wild sesame seeds after 3 weeks of powdering and Guangdong Yazhou black seeds from 2 weeks after powdering were soaked in 70% ethanol for half a minute and then surface sterilized with 0.1% HgCl2 for 12 minutes. The embryos were removed from the seedbed on a clean bench and inoculated into culture medium: (1) LSM + 0.2mg / L 6-BA + 2% sucrose, pH 5.6, (2) LSM + 0.2mg / BA + sucrose 2% + hydrolyzed milk protein 600 mg / L, pH 5.6. At 25-29 ℃, light 12 hours a day