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对 7例原发性中枢神经系统淋巴瘤 (Primarycentralnervoussystemlymphoma ,PCNSL)进行免疫组化及病理形态定量研究。方法 对瘤组织采用常规石蜡包埋切片、HE染色及LSAB免疫组化染色 ,光镜观察。另选取病变典型的切片应用HPIAS - 10 0 0彩色图像分析系统对肿瘤细胞核的形态进行定量测定。结果 7例均为B细胞淋巴瘤 ,共中大裂 -无裂细胞型 4例 ,小淋巴细胞型 2例 ,小裂细胞型 1例。各型淋巴瘤与对照组之间除椭圆度外 ,其余形态学参数均有非常显著性差异 (P <0 .0 0 1)。 3组淋巴瘤两两间比较显示大裂 -无裂细胞型与小裂细胞型、大裂 -无裂细胞型与小淋巴细胞型除个别指标、多数参数两两间有非常显著性 (P <0 .0 0 1)或显著性差异 (P <0 .0 5) ;小淋巴细胞型与小裂细胞型间 ,面积等效体积、等效圆直径、长径、短径、形状因子、矩形度、实面积 7项参数有显著性差异。其余 8项参数无显著性差异。结论 采用形态定量的方法能对PCNSL做出准确的诊断 ,对于PCNSL的某些组织学分型能够提供客观的、准确的和定量的依据 ,因而具有十分重要的意义。
Seven cases of primary central nervous system lymphoma (Primary central nervous system lymphoma, PCNSL) immunohistochemistry and pathological morphology quantitative study. Methods Tumor specimens were embedded in paraffin embedded sections, HE staining and LSAB immunohistochemical staining and light microscopy. In addition, a typical section of the lesion was selected for quantitative determination of the morphology of tumor nuclei using the HPIAS-1000 color image analysis system. Results All the 7 cases were B-cell lymphoma. There were 4 cases of common split-uncrack cell type, 2 cases of small lymphocyte type and 1 case of small cell type. In addition to ovality between the various types of lymphoma and the control group, the remaining morphological parameters were very significant differences (P <0. In the 3 groups of lymphoma, comparisons between the two groups showed that there was a significant difference between the two groups (P> 0 0 01) or significant difference (P 0 05); between small lymphocyte type and small cleaved cell type, equivalent volume of area, equivalent circle diameter, long diameter, short diameter, shape factor, rectangle Degree, real area 7 parameters have significant differences. The remaining 8 parameters no significant difference. Conclusion The method of morphometric quantification can make accurate diagnosis of PCNSL. It is of great significance for some histological types of PCNSL to provide objective, accurate and quantitative evidence.