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目的：观察胰腺和癌变标本中血小板活化生长因子ＰＤＧＦ及其受体ＰＤＧＦＲ基因表达的变化关系，探讨ＰＤＧＦ及其ＰＤＧＦＲ在肿瘤侵袭和转移过程中的作用。方法：用Ｎｏｒｔｈｅｒｎ杂交方法，采用ｃＤＮＡ探针（ＰＤＧＦＡ、Ｂ，ＰＤＧＦＲ－α、－β）对两株胰腺癌细胞（ＰａＴｕ８９８８、ＰａＴｕ８９０２）、１５例胰腺癌手术切除标本和８例正常胰腺组织标本进行了分子杂交检测。结果：正常胰腺组织中，在ＲＮＡ水平，ＰＤＧＦＢ检测不出；ＰＤＧＦＡ及其ＰＤＧＦＲ－β有相对高的表达水平，ＰＤＧＦＲ－α的表达水平相对较低；趋势为ＰＤＧＦＡ＞ＰＤＧＦＲ－β＞ＰＤＧＦＲ－α。胰腺癌组织中，ＰＤＧＦＡ、ＰＤＧＦＢ在ＲＮＡ水平的表达与正常胰腺组织相近；而ＰＤＧＦＲ－α、ＰＤＧＦＲ－β在ＲＮＡ水平的表达，尤其是ＰＤＧＦＲ－α的明显表达较正常胰腺组织相对提高。ＰａＴｕ细胞株中，有ＰＤＧＦＡ及其ＰＤＧＦＲ－β的表达。结论：ＰＤＧＦ、ＰＤＧＦＲ可能通过对胰腺癌细胞外基质降解代谢的调控，共同影响着肿瘤的侵袭和转移。
OBJECTIVE: To observe the changes of platelet activating factor PDGF and its receptor PDGFR gene expression in pancreatic and cancerous specimens, and to explore the role of PDGF and its PDGFR in tumor invasion and metastasis. METHODS: Two pancreatic cancer cells (PaTu8988, PaTu8902), 15 surgical specimens of pancreatic cancer and 8 normal pancreatic tissue specimens were studied by Northern hybridization using cDNA probes (PDGFA, B, PDGFR-α, -β). Molecular hybridization assays. RESULTS: In normal pancreatic tissues, PDGFB was not detected at the RNA level; PDGFA and PDGFR-beta had relatively high expression levels; PDGFR-alpha expression was relatively low; the trend was PDGFA> PDGFR-beta> PDGFR-alpha . In pancreatic cancer tissues, the expression of PDGFA and PDGFB at the RNA level was similar to that of normal pancreatic tissue. The expression of PDGFR-α and PDGFR-β at the RNA level, especially the expression of PDGFR-α, was relatively higher than that of normal pancreatic tissue. In PaTu cell line, PDGFA and its PDGFR-β expression. Conclusion: PDGF and PDGFR may affect the invasion and metastasis of tumors through the regulation of the degradation and metabolism of extracellular matrix of pancreatic cancer cells.