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采用蕾期剥蕾授粉的方法,成功获得了新疆野生油菜野油2000-1和甘蓝型油菜Parol属间杂种。杂种F1育性极低,自由授粉条件下获得少量F2种子。在F2群体中成功选择到一株雄性不育株,用Parol做轮回亲本与不育株回交,BC1、BC2和BC3群体中可育株与不育株均呈1∶1分离。用波里马细胞质雄性不育的保持系和恢复系、681A的保持系和恢复系、隐性核不育86A的保持系及其它33个甘蓝型油菜品系与BC2群体中的不育株测交,测交后代可育株与不育株呈5∶3分离的组合有20个,呈1∶1分离的组合有14个,呈3∶1分离的组合有3个,有一个组合的后代完全可育,表明该雄性不育材料可能为显性核不育。
By bud-pollination method, we successfully obtained Xinjiang wild rapeseed wild oil 2000-1 and Brassica napus Parol interspecific hybrids. Cross F1 fertility is extremely low, a small amount of F2 seeds are obtained under free pollination conditions. One male sterile line was successfully selected in F2 population. The backcross with Parol was backcrossed with the sterile line. The male sterile line and the sterile line in BC1, BC2 and BC3 populations all showed 1: 1 segregation. The maintainer line and restorer line of cytoplasmic male sterility in Polima, the maintainer line and restorer line of 681A, the maintainer line of recessive nuclear sterility 86A and the other 33 lines of Brassica napus were crossed with the sterile line of BC2 , There were 20 combinations of 5: 3 fertile plants and sterile plants measured in the offspring, 14 in 1: 1 separation, 3 in 3: 1 separation, and one in combination Fertile, indicating that the male sterile material may be dominant nuclear infertility.