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为了改变传统的Golgi-Cox染色方法,使其在纹状体神经元形态与结构的研究中更加稳定和有效,本实验将昆明小鼠随机分为两组,一组采用传统的Golgi-Cox染色法,另一组采用改良的Golgi-Cox染色法。改良方法在传统方法的基础上,改变了几个关键环节,包括溶液的配制、固定、包埋、切片和定影等,然后对两种方法进行统计学比较和分析。在所有的数据采集工作完成之前,切片上的标记是封闭的。通过统计学分析比较,发现改良方法能够稳定地显示更多的树突分支(增加50%)、树突棘(增加63%)和胞体(增加一倍)。改良的Golgi-Cox染色方法比传统的Golgi-Cox染色方法更加稳定和敏感,在纹状体神经元树突和树突棘形态与结构研究中是一种可靠的技术方法。
In order to change the traditional Golgi-Cox staining method to make it more stable and effective in the study of the morphology and structure of striatal neurons, Kunming mice were randomly divided into two groups. One group was treated with the conventional Golgi-Cox staining Method, the other group using a modified Golgi-Cox staining. Based on the traditional method, the improved method changes several key steps, including the solution preparation, fixation, embedding, sectioning and fixing, and then compares and analyzes the two methods statistically. The mark on the slice is closed until all data collection is complete. By statistical analysis and comparison, it was found that the improved method could stably show more dendritic branches (increased by 50%), dendritic spines (increased by 63%) and soma (doubling). The improved Golgi-Cox staining method is more stable and sensitive than the traditional Golgi-Cox staining method and is a reliable technique in the study of the morphology and structure of dendrites and dendritic spines of striatal neurons.